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Image Search Results
Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Phosphopeptide Enrichment Using Offline Titanium Dioxide Columns for Phosphoproteomics
doi: 10.1007/978-1-62703-360-2_8
Figure Lengend Snippet: The principle of assembling the TiO2 cartridge column, setup of the syringe pump, and phosphopeptide enrichment. (A) A TiO2 column, a cartridge holder, 1/16” OD PEEK tubing, 1/16” OD fittings, a Rheodyne® needle port adapter, a polymer tubing cutter, and 2-3 syringes are needed to assemble the TiO2 cartridge column device for phosphopeptide enrichment. (B) The TiO2 column is assembled in a cartridge holder by finger-tightening. (C) A ~2 inches of 1/16” OD PEEK tube is cut using a polymer tubing cutter. (D) Insert the tube into a 1/16” ferrule, and connect the tube to the outlet of the TiO2 column using a 1/16” nut by finger-tightening. (E) Connect the Rheodyne® needle port adapter to the inlet the TiO2 column by finger-tightening. (F) Insert the needle of a syringe into the needle port adapter of the assembled TiO2 cartridge column and place them onto the syringe pump. The device is ready for phosphopeptide enrichment. (G) Schematic diagram showing the principle of TiO2 enrichment of phosphopeptides with a column wash of 200 mM NH4Glu, pH 2.0.
Article Snippet: Syringes: 1-2 syringes of 5 mL (22 gauge needle) capacity for column washing, 2-3 syringes of 2.5 mL (22 gauge needle) capacity for sample loading, column washing, and
Techniques: Polymer